SOP for
Operation of Refractometer
1.0
Purpose
The purpose of
this SOP is to lay down operational procedure of Refractometer.
2.0
Scope
This procedure is applicable to Quality Control
Department.
3.0
Responsibility
Officer QC
Asst. Manager QC
Manager QC
4.0
Accountability
Manager QA
5.0
Terms and Definitions
N/A
6.0
References
N/A
7.0
Precautions
7.1
Wear mask and
gloves while operating the instrument.
7.2
Operate instrument
carefully
7.3 Keenly observe
temperature as temperature variation significantly change refractive index.
8.0
Procedure
8.1
Ensure that the
instrument and area is properly clean and free from dust.
8.2
Place instrument
such that proper light can be reflected through the mirror.
8.3 Unlock the prism
and clean its surface using distilled water or naphthalene bromide with the
help of tissue paper.
8.4
Place few drops
of sample testing and allow it to smear on the prism surface.
8.5
Quickly close the
prism lock.
8.6
Adjust the light
using shield fixed below the prism.
8.7 Adjust the eye
piece cross wire with a knob by rotating it in a clockwise or anti-clockwise
direction so that the sharp demarcation line passes through its center.
8.7.1 Observe sharp
demarcation produced in circle form having dark and light portions equally.
8.8 Use a knob
present just above the prism for fine adjustment.
8.9 Observe and
record the reading using magnifying lens of the instrument from the scale
8.10
Dry & clean the
instrument with the help of tissue paper and close the knob.
9.0
Abbreviations
9.1
SOP Standard Operating Procedure
9.2
QC Quality Control
9.3
QA Quality Assurance
9.4
Asst Assistant
10.0
Annexure
N.A
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