Moxifloxacin as HCl Quantitative Analysis On UV Visible Spectrophotomete

 

Moxifloxacin as HCl Quantitative Analysis On UV Visible Spectrophotomete

Moxifloxacin as HCl Quantitative Analysis On UV Visible Spectrophotomete

¨  By Applying the below method of testing of Moxifloxacin as HCl we can Analyze active raw material, and different formulated dosage forms.

¨  Absorbance will be measured at  295 nm using UV-Visible Spectrophotometer.

¨  Solvent Used 0.1 M HCl

Standard Preparation:

¨  Accurately weight Moxifloxacin as HCl working standard 50mg using butter paper       and transfer it to a 50ml volumetric flask.

¨  Add 20ml of 0.1M HCl and sonicate for 10 minutes. Then make up the volume with 0.1M HCl up to 50ml.

¨  Further take 1 ml of this solution into 50ml volumetric flask and make up the volume up to 50ml with 0.1M HCl .

Sample Preparation:

¨  Accurately weight sample equivalent to 50 mg of Moxifloxacin as HCl and transfer it to a 50ml volumetric flask.

¨  Add 20ml of 0.1M HCl , first sonicate for 10 minutes and then stir for further 10 minutes. Make up the volume with 0.1M HCl up to 50ml.

¨  Filter the sample through whatman filter paper.

¨  Further take 1ml of this solution into 50ml volumetric flask and make up the volume up to 50ml with 0.1M HCl .

¨  In case of granules, pellets and tablets it is necessary to crush the sample and make fine powder sample for better solubility.

¨  Measure the absorbance of both sample and standard solutions in a 1 cm quartz cell on a U.V/ Visible spectrophotometer at 295 nm.

¨  Calculation:

%age = Abs. of sample  x conc. of stand x 100

                         Abs. Standard  x conc. of sample