Calibration of Pipette

 

Calibration of Pipette

Calibration of Pipette

¨  Take already washed, dried and cool beaker and put it on the balance and tare it.

¨  Take Distilled water in another beaker and record its temperature using calibrated thermometer.

¨  Take an already washed, cleaned, dried and cooled pipette and fill it with Distilled water accurately up to the mark.

¨  Transfer the whole volume of Distilled water to the beaker

¨  Note down the weight of distilled water as “W”

¨  Now determine the volume in ml using following formula

¨  Volume (in ml) = weight (in gm.)

¨                                            0.997

¨  0.997 is density of distilled water at 25^oC.

¨  Repeat the process for 3 times to attain consistent results.

¨  Variation in volume can be calculate using following equation

¨  Volume Variation (+ml) = Theoretical volume – Actual volume