Analysis of Aceclofenac Raw Material and Dosage Form Using UV Visible Spectrophotometer

 

Analysis of Aceclofenac  Raw Material and Dosage Form Using UV Visible Spectrophotometer

Analysis of Aceclofenac  Raw Material and Dosage Form Using UV Visible Spectrophotometer

¨  Aceclofenac Raw material and dosage from can be analyze quantitatively as follow.

¨  Absorbance will be measured at  275 nm using UV-Visible Spectrophotometer.

Standard Preparation:

¨  Accurately weight Aceclofenac working standard 25mg using butter paper and transfer it to a 50ml volumetric flask.

¨  Add 20ml of methanol and sonicate for 10 minutes. Allow to cool and make up the volume with methanol up to 50ml.

¨  Further take 1 ml of this solution into 50ml volumetric flask and make up the volume up to 50ml with methanol.

Sample Preparation:

¨  Accurately weight sample equivalent to 25 mg of Aceclofenac using butter paper and transfer it to a 50ml volumetric flask.

¨  Add 20ml of methanol first sonicate for 10 minutes and then stir for further 10 minutes. Allow to cool and make up the volume with methanol up to 50ml.

¨  Filter the sample through whatman filter paper.

¨  Further take 1 ml of this solution into 50ml volumetric flask and make up the volume upto 50ml with methanol.

¨  In case of granules, pellets and tablets it is necessary to crush the sample and make fine powder sample for better solubility.

¨  Measure the absorbance of both sample and standard solutions in a 1 cm quartz cell on a U.V/ Visible spectrophotometer at 275 nm.

¨  Calculation:

¨  %age = Abs. of sample  x conc. of stand x 100

                         Abs. Standard  x conc. of sample