0.05 Molar EDTA Solution Preparations and Standardization

 

0.05 Molar EDTA Solution Preparations and Standardization

0.05 Molar EDTA Solution Preparations

¨  To prepare 0.05 molar Edetate disodium solution accurately weigh 18.6 gram Edetate disodium and dissolve in sufficient carbon-dioxide free water.

¨  Make the final volume up to 1000 ml with carbon-dioxide free water.

¨  Formula used

¨  Mass in gram= Molarity x molecular weight x required volume

                                                             1000

¨  Mass in gram = 0.05 x 372.24 x 1000     = 18.6 g

                                                    1000

Standardization of 0.05 molar EDTA solution

¨  Accurately weight about 200mg calcium carbonate.

¨  Dried at 110◦C for 2 hours in an oven and then cooled in desiccator.

¨  Transfer it into a conical flask of about 500 ml and add 10 ml of carbon-dioxide free gently with constant stirring to form slurry.

¨  Add 2 ml of dilute HCl with proper shaking to dissolve calcium carbonate.

¨  Further add 100 ml of carbon-dioxide free water with constant stirring using magnetic stirrer.

¨  Now add 30 ml 0.05 Molar EDTA solution (freshly prepared) with the help of burette gently.

¨  Add 15 ml of sodium hydroxide TS.

¨  Further add 300 mg of Hydroxy Naphthol blue indicator.

¨  Titrate with 0.05 Molar Edetate disodium solution and find the molarity as follow

 

 

¨  Molarity     =     weight of calcium carbonate x 1000

                 Molecular weight of Calcium carbonate x volume used of 0.05 M EDTA

 

¨  Molarity    =       weight of CaCO3 x 1000

                            100.09 x Volume used of 0.05M EDTA solution

 

 Presented By

Muhammad Atif

PhD Scholar Pharmaceutical Sciences

Abdul Wali Khan University Mardan, Pakistan