Vancomycin HCl
quantitative Analysis On UV Visible Spectrophotometer
Active Raw Material & Dosage Forms
¨ By
Applying the below method of testing of Vancomycin HCl we can Analyze active
raw material, and different formulated dosage forms.
¨ Absorbance
will be measured at 280 nm using
UV-Visible Spectrophotometer.
¨ Solvent
Used Distilled Water
¨ Standard
Preparation: Accurately weight Vancomycin HCl working standard 50mg using
butter paper and transfer it to a 50ml volumetric flask.
¨ Add
20ml of Distilled Water and sonicate for 10 minutes. Then make up the volume
with Distilled water up to 50ml.
¨ Further
take 5 ml of this solution into 25ml volumetric flask and make up the volume up
to 25ml with Distilled water.
¨ Sample
Preparation: Accurately weight sample equivalent to 50 mg of Vancomycin HCl
and transfer it to a 50ml volumetric flask.
¨ Add
20ml of Distilled water, first sonicate for 10 minutes and then stir for
further 10 minutes. Make up the volume with Distilled water up to 50ml.
¨ Filter
the sample through whatman filter paper.
¨ Further
take 5 ml of this solution into 25ml volumetric flask and make up the volume up
to 25ml with Distilled water.
¨ In
case of granules, pellets and tablets it is necessary to crush the sample and
make fine powder sample foe better solubility.
¨ Measure
the absorbance of both sample and standard solutions in a 1 cm quartz cell on a
U.V/ Visible spectrophotometer at 280 nm.
¨ Calculation:
¨ %age
= Abs. of sample x conc. of stand x
100
Abs. Standard x conc. of sample
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