Standardization of 1 Molar Hydrochloric acid solution

 



Standardization of 1 Molar Hydrochloric acid solution

Standardization of 1 Molar Hydrochloric acid solution

¨  Accurately weigh about 5.0 gram (5000mg)of tromethamine.

¨  Dried as per instructions given on label claim, however if no such instructions are available then dried for 3 hours at 105◦C in oven.

¨  Dissolve in 50ml of carbon-dioxide free water.

¨  Add 2 drops of bromocresol indicator.

¨  Titrate with 1 molar Hydrochloric acid with constant stirring until a pale yellow color is produced.

¨  Each ml of 1 molar Hydrochloric acid is equivalent to 121.14 mg of tromethamine.

¨  Calculate the molarity using following formula

¨  Molarity=       weight of tromethamine taken

                   molecular weight of tromethamine x ml of 1 M HCl used

¨  Molarity= weight of tromethamine taken

                            121.14 x ml of 1 M HCl used

OR

¨  Accurately weight 1 gram (1000mg)of anhydrous Sodium carbonate and dried is as per the instructions given on the label.

¨  However if no such instructions are available then dried the Sodium carbonate for 1 hour at 270◦C.

¨  Dissolve it in 100 ml of carbon-dioxide free water.

¨  Add 0.1 ml of methyl orange solution.

¨  Titrate with 1 molar HCl solution with constant stirring until the solution becomes reddish yellow.

¨  Boil it for 2 minutes and continue the titration with constant stirring until the reddish yellow color restored.

¨  Each ml of 1 molar hydrochloric acid is equivalent to 52.99 mg of Sodium carbonate anhydrous.

¨   

¨  Molarity  =                   weight of anhydrous sodium carbonate taken

                                         molecular weight of sodium carbonate anhydrous x ml of 1 M HCl used

 

¨  Molarity=   weight of anhydrous sodium carbonate taken

                                           52.99 x ml of 1 M HCl used

Note

¨  Standardization is only required when the solution is used for quantitative analysis.

¨  In case of qualitative analysis such as pH adjustment, dissolution medium or diluent there is no need of standardization.

 

 Presented By

Muhammad Atif

PhD Scholar Pharmaceutical Sciences 

Abdul Wali Khan University Mardan, Pakistan

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