omeprazole
quantitative Analysis On UV Visible Spectrophotometer
Active Raw Material & Dosage Forms
¨ By
Applying the below method of testing of Omperazole we can Analyze active raw
material, and different formulated dosage forms.
¨ Absorbance
will be measured at 305 nm using
UV-Visible Spectrophotometer.
¨ Solvent
Used 0.1 M NaOH
¨ Standard
Preparation: Accurately weight Omperazole working standard 50mg using
butter paper and transfer it to a 100ml volumetric flask.
¨ Add
20ml of 0.1M NaOH and sonicate for 10 minutes. Then make up the volume with
0.1M NaOH up to 100ml.
¨ Further
take 1 ml of this solution into 50ml volumetric flask and make up the volume up
to 50ml with 0.1M NaOH .
¨ Sample
Preparation: Accurately weight sample equivalent to 50 mg of Omeprazole and
transfer it to a 100ml volumetric flask.
¨ Add
20ml of 0.1M NaOH , first sonicate for 10 minutes and then stir for further 10
minutes. Make up the volume with 0.1M NaOH up to 100ml.
¨ Filter
the sample through whatman filter paper.
¨ Further
take 1ml of this solution into 50ml volumetric flask and make up the volume up
to 50ml with 0.1M NaOH .
¨ In
case of granules, pellets and tablets it is necessary to crush the sample and
make fine powder sample to attain better solubility.
¨ Measure
the absorbance of both sample and standard solutions in a 1 cm quartz cell on a
U.V/ Visible spectrophotometer at 305 nm.
¨ Calculation:
¨ %age
= Abs. of sample x conc. of stand x
100
¨ Abs. Standard x conc. of sample
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