Loratadine Quantitative Analysis On UV Visible Spectrophotometer

 

Loratadine Quantitative Analysis On UV Visible Spectrophotometer

Loratadine Quantitative Analysis On UV Visible Spectrophotometer

¨  By Applying the below method of testing of Loratadine we can Analyze active raw material, and different formulated dosage forms.

¨  Absorbance will be measured at  280 nm using UV-Visible Spectrophotometer.

¨  Solvent Used 0.1 M HCl

Standard Preparation: Accurately weight Loratadine working standard 50mg using butter paper and transfer it to a 50ml volumetric flask.

¨  Add 20ml of 0.1M HCl and sonicate for 10 minutes. Then make up the volume with 0.1M HCl up to 50ml.

¨  Further take 1 ml of this solution into 100ml volumetric flask and make up the volume up to 100ml with 0.1M HCl .

Sample Preparation: Accurately weight sample equivalent to 50 mg of Loratadine and transfer it to a 50ml volumetric flask.

¨  Add 20ml of 0.1M HCl , first sonicate for 10 minutes and then stir for further 10 minutes. Make up the volume with 0.1M HCl up to 50ml.

¨  Filter the sample through whatman filter paper.

¨  Further take 1ml of this solution into 100ml volumetric flask and make up the volume up to 100ml with 0.1M HCl .

¨  In case of granules, pellets and tablets it is necessary to crush the sample and make fine powder sample foe better solubility.

¨  Measure the absorbance of both sample and standard solutions in a 1 cm quartz cell on a U.V/ Visible spectrophotometer at 280 nm.

¨  Calculation:

¨  %age = Abs. of sample  x conc. of stand x 100

                          Abs. Standard  x conc. of sample

 

 Presented by

Muhammad Atif

PhD Scholar Pharmaceutical Sciences 

Abdul Wali Khan University Mardan, Pakistan