Ebastine
quantitative Analysis On UV Visible Spectrophotometer
Active Raw Material & Dosage Forms
¨ By
Applying the below method of testing of Ebastine we can Analyze active raw
material, and different formulated dosage forms.
¨ Absorbance
will be measured at 252 nm using
UV-Visible Spectrophotometer.
¨ Solvent
Used Methanol
¨ Standard
Preparation: Accurately weight Ebastine working standard 50mg using butter
paper and transfer it to a 50ml volumetric flask.
¨ Add
20ml of Methanol and sonicate for 10 minutes. Then make up the volume with
Methanol up to 50ml.
¨ Further
take 1 ml of this solution into 100ml volumetric flask and make up the volume
up to 100ml with Methanol.
¨ Sample
Preparation: Accurately weight sample equivalent to 50 mg of Ebastine and
transfer it to a 50ml volumetric flask.
¨ Add
20ml of Methanol, first sonicate for 10 minutes and then stir for further 10
minutes. Make up the volume with Methanol up to 50ml.
¨ Filter
the sample through whatman filter paper.
¨ Further
take 1ml of this solution into 100ml volumetric flask and make up the volume up
to 50ml with Methanol.
¨ In
case of granules, pellets and tablets it is necessary to crush the sample and
make fine powder sample foe better solubility.
¨ Measure
the absorbance of both sample and standard solutions in a 1 cm quartz cell on a
U.V/ Visible spectrophotometer at 252 nm.
¨ Calculation:
¨ %age
= Abs. of sample x conc. of stand x
100
¨ Abs. Standard x conc. of sample
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